Acceleration of amyloid fibril formation by multichannel sonochemical reactor

Abstract The formation of amyloid fibrils various amyloidogenic proteins is dramatically enhanced by ultrasound irradiation. To apply this phenomenon to the study protein aggregation science and diagnosis neurodegenerative diseases, a multichannel irradiation system with individually adjustable conditions necessary. Here, we develop sonochemical reaction system, where an ultrasonic transducer placed in each well 96-well microplate perform sample solutions under high reproducibility, applied it fibril ? , ? -synuclein, 2-microglobulin, lysozyme. results clearly show that our instrument superior conventional shaking method terms degree acceleration reproducibility reaction. controllable controlling driving voltage transducer. We have thus succeeded developing useful tool for proteins.